Human‐Derived Induced Pluripotent Stem Cells‐Based Disease Models of the Alzheimer’s Disease (AD) Patients Expressed AD Phenotypes and Responded to 17β‐Estradiol Treatment

Background Neuronal models of both familial and sporadic cases Alzheimer’s Disease (fAD/sAD) were generated from the human-derived induced pluripotent stem cells (iPSCs) AD patients. Using this in vitro cellular models, AD-like phenotypes was analyzed with or without 17β-estradiol, sex hormone that are supposed to be involved pathogenesis. Method 5 lines iPSCs comprised 1 healthy control, 2 fAD, sAD differentiated neurons a newly established neuronal differentiation method. The such as secreted Aβ peptides excitability measured by ELISA Ca-imaging (Fluo-8 indicator). In addition, complexity examined after long (4 days) short (15 minutes) 17β-estradiol treatment, respectively. Result successfully more Aβ(1-40) Aβ(1-42) increased Ca oscillations compared control line. treatment promoted excitability. Conclusion Neurons derived patients expressed responded 17β-estradiol. Thus, suggested therapeutic effects steroid hormones can evaluated human cells. This enables us examine variability estradiol response among plan clinical trials based on responsiveness.